Fig. 4

Effects of bafilomycin A1 treatment on AgNP-induced perturbation of lysosomes. A549 cells were incubated in culture medium with or without 200 nM bafilomycin A1 (BFA1) for 24 h before treating with or without AgNPs (50 μg Ag/ml) for an additional 24 h. Then, cells were incubated with LysoTracker for 30 min. The lysosomes of cells were visualized by confocal laser microscopy with LysoTracker staining (green signal). DIC and fluorescence images were merged (merge). Scale bar, 10 μm