Fig. 1
From: Improved method for measurement of multi-walled carbon nanotubes in rat lung
A newly developed for MWCNT. a Lung tissue with the C99 at room temperature. b Lung digestion in C99. c Centrifugation of lung solution at 12,000 rpm for 10 min. d Supernatant removal and 60 % sulfuric acid addition to MWCNT samples. e MWCNT-marker hybrid method: Marker adsorbtion onto MWCNT for 15 min. f MWCNTs adhering to Nuclepore membrane filters were extracted with marker on MWCNTs. g Filter with MWCNT was then punched out in circular disks h Addition of 1 ml acetonitrile, stirring, followed by the extraction of the marker in MWCNTs and the acetonitrile filtration of marker in the MWCNTs. i HPLC analysis