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Table 1 Program of time controlled steps for the LC gradient pump and the six-port switching valve.

From: Method optimization and validation for the simultaneous determination of arachidonic acid metabolites in exhaled breath condensate by liquid chromatography-electrospray ionization tandem mass spectrometry

Time (min)

Flow (mL/min)

Solvent A (%)

Solvent B (%)

Valve Position

0

0.25

70

30

A

5

0.25

70

30

B

11

0.25

40

60

 

12

0.25

0

100

 

17

0.25

0

100

 

19

0.25

70

30

A

21

0.25

70

30

 
  1. Solvent A: Ammonium Acetate buffer 2 mM (pH 4.6)/Acetonitrile (99.5/0.5 v/v)
  2. Solvent B: Ammonium Acetate buffer 2 mM/Acetonitrile/glacial acetic acid (2/97/1; v/v)
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Journal of Occupational Medicine and Toxicology

ISSN: 1745-6673