Table 3 Reliability data of the method for the determination of eicosanoids in exhaled breath condensate.
Analyte | Intra-day precision | Inter-day precision | Accuracy | Calibration (Y = ax+b) | ||||
|---|---|---|---|---|---|---|---|---|
Q1 | Q2 | Q1 | Q2 | relative recovery (%) | a (x10e-3) | b (x10e-3) | ||
RSD(%) | RSD(%) | RSD(%) | RSD(%) | mean | range | |||
8-iso-PGF 2alfa | 5 | 5 | 16 | 11 | 94 | 88–107 | 0,098 | 2,21 |
11-β-PGF 2alfa | 4 | 2 | 10 | 8 | 102 | 97–108 | 0,084 | 0,358 |
PGF 2alfa | 2 | 2 | 13 | 8 | 102 | 99–104 | 0,254 | 0,314 |
PGE 2 | 4 | 2 | 5 | 6 | 99 | 96–107 | 0,628 | 1,92 |
PGD 2 | 4 | 2 | 4 | 6 | 102 | 94–112 | 0,345 | 0,089 |
13,14-dihydro-15-keto-PGE 2 | 2 | 2 | 8 | 8 | 94 | 90–100 | 1,46 | -0,245 |
13,14-dihydro-15-keto-PGD 2 | 2 | 3 | 9 | 8 | 104 | 99–110 | 1,04 | 4,88 |
LTE 4 | 6 | 4 | 10 | 7 | 115 | 96–133 | 0,238 | 1,3 |
Delta 12-PGJ 2 | 5 | 5 | 13 | 11 | 105 | 98–120 | 1,09 | 1,2 |
PGJ 2 | 5 | 6 | 4 | 8 | 110 | 102–119 | 0,213 | 1,17 |
LTB 4 | 5 | 5 | 9 | 11 | 110 | 97–117 | 0,19 | 1,4 |
15-desoxy-delta12,14-PGJ 2 | 5 | 5 | 16 | 12 | 159 | 135–179 | 0,739 | 5,46 |