Figure 6
From: Lung responses to secondary endotoxin challenge in rats exposed to pig barn air
TGF-β2 expression and quantification in the lung. Immunohistochemical expression of TGF-β2 was detected using anti- TGF-β2 antibody in the lung sections from controls (A-B), one day (C-D) exposed rats without and with E. coli LPS challenge respectively, five day exposed rats without E. coli LPS challenge (E) and with E. coli LPS challenge (picture not shown). TGF-β2 expression in the airway epithelium (arrows, A-E) is shown. F. Quantification of TGF-β2 protein using ELISA showed increased levels in control rats without E. coli LPS challenge compared to control rats with E. coli LPS challenge (*, P = 0.001).Original magnification A-F: ×400 and micrometer bar = 50 μm.