Figure 6

Western blot analysis of Cx43, α-tublin, and E-cadherin protein expression alterations in TPTC treated WB F344 liver cells. A: T, TPTC 1.5 ppm (15-min exposure); D, negative control (DMSO). B: T, TPTC 1.5 ppm (30-min exposure); D, negative control (DMSO). P0 gradually decreased and density of P1 increased. The band of P0 totally disappeared after exposure to 1.5 ppm TPTC for 30 min. No change of α-tublin, and E-cadherin protein were found. MW: Connexin 43 was 43 kD, α-Tublin was 55 kD, and E-Cadherin was 120 kD.