Time-dependent effects of different Roundup formulations on HepG2 and Hep3B cell viability. The formulations were applied during 24 h (A and C) or 48 h (B and D). These effects were evaluated by the MTT test (see Methods), measuring mitochondrial succinate dehydrogenase activity. The results are presented in percent compared to non treated cells (M). Cells were grown at 37°C (5% C02, 95% air) in medium EMEM with 10% serum during 48 h to 80% confluence in 24-well plates, and then exposed to 4 different Roundup formulations. On × axis, concentrations of G in R in parenthesis: Express® 7.2 g/l of G, Bioforce® 360 g/l of G, GT® 400 g/l of G, GT+® 450 g/l of G, all at 0.5%. All experiments were repeated 3 times in triplicates. Statistically significant differences are calculated in comparison to control by a student t-test p < 0.01(**) and p < 0.05(*).