Figure 3

Dig1 general preventive effect of R400 toxicity on Hep3B and HepG2 during 72 h. In frames on the right, each letter (M, R or D) indicates 24 h of successive cell exposures to the corresponding conditions (Medium alone, Roundup, Dig1). The results were evaluated as in Fig. (1). To measure preventive effects, D at 2% was applied during 24 or 48 h before R (400 g/l at LC50 in these conditions, 40 ppm for Hep3B and 96 ppm for HepG2): corresponding treatments called DRM or DDR. R was applied alone as negative control during 24 or 72 h (RMM, RRR), or before D to assess curative effects (RDM, RMD or RDD), all these curves are superimposed, and thus only RMM is shown as negative control. No curative effect is evidenced in these conditions. Efficiencies of protection (EP) after 24 or 48 h of treatment by D are indicated in frames.