Figure 4

¹⁴C-glyphosate entered in HepG2 cells in presence or absence of Roundup and Dig1 or Dig2. Cells were grown in 24-well plates and other conditions and calculations are explained in figure 1. ¹⁴C-Glyphosate (G*) entry in cells was measured in % by counting the radioactivity in pellets, and taking into account the mortality (20%) according to the formula [G* in pellets × (120/100)] × 100/G* in supernatants. The treatments were changed every 24 h, cells were treated 48 h: for instance, medium (M) for the first day and G* for the second day will be indicated M/G*. G* is at a final concentration of 0.266 μmol/ml for a specific activity of 55 μCi/μmol, corresponding to the quantity of G in 0.01% of R (nontoxic in these conditions). Also, G* was a tracer in a 0.01% dilution of R (RG*), including the adjuvants; the G final concentration was still nontoxic. Dig1 (D) or Dig2 (D2) at 2% or medium alone (M) were incubated during the first or the second day to study preventive or curative effects. The only statistical differences between two treatments are indicated by ** (p < 0.01).