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Figure 7 | Journal of Occupational Medicine and Toxicology

Figure 7

From: Immune sensitization to methylene diphenyl diisocyanate (MDI) resulting from skin exposure: albumin as a carrier protein connecting skin exposure to subsequent respiratory responses

Figure 7

Identification of MDI antigens in urea extracts of exposed skin. (A) The detergent insoluble fraction of (-) control or (+) 10% MDI exposed skin tissue were further homogenized in 9 M urea, separated by SDS-PAGE, and stained for total proteins (lanes 1 and 2). Parallel Western blot with sera from autologous MDI skin exposed mice (lanes 3 and 4) vs. control mouse sera (lanes 5 and 6) identified at least three antigenically modified proteins (MDI antigens) in these samples; see arrows. (B) The MDI antigens from 10% MDI exposed mouse skin were purified and reanalyzed by protein stain following SDS-PAGE, and parallel Western blot with autologous sera from MDI skin exposed mice. Arrows highlight antigenically modified collagen (*1), keratin (*2) and tropomyosin (*3) from MDI exposed skin. Actin from unexposed mouse skin, which was not recognized by autologous sera, was run as a negative control (lane 3). MDI antigens were not detectable using control sera from vehicle expose mice or irrelevant hyperimmune mouse serum (not shown).

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