Human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development

Table 1 The effect of coculture on development of mouse embryos in increasing Bisphenol A

Concentrations (M)	Embryos grown on*	Total number of embryos	Number of blastocysts	% of blastocyst ^a
0	Medium alone	125	116	93
	Vehicle	144	130	90
	Coculture	112	110	98^b
10⁻⁸	Medium alone	121	102	84
	Vehicle	132	107	81
	Coculture	123	111	90^b
10⁻⁶	Medium alone	120	80	67
	Vehicle	123	76	62
	Coculture	121	87	72^b
10⁻⁴	Medium alone	135	0	0^c
10⁻⁴	Vehicle	113	0	0^c
	Coculture	123	62	50^bc

*Mouse embryos were grown on three different kinds of culture system. In Medium alone M199 (Gibco) media were used. In Vehicle, vehicle solutions (ethanol/methanol) were used. In co-culture, embryos were grown on human endometrial epithelial cells (hEECs).
^aThe percentages of blastocyst at 72hour of culture.
^bValues in coculture were significantly higher than those in medium alone, respectively: P < 0.05.
^cValues were significantly lower than those of each control(0 μg/ml), respectively: P < 0.05.

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