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Table 2 The effect of coculture on development of mouse embryos in increasing Aroclor 1254 concentrations

From: Human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development

Concentrations

          (μg/ml)fs

Group

Total number of embryos

Number of blastocysts

% of

blastocyst a

0

Medium alone

122

111

91

Vehicle

113

102

90

Coculture

133

125

94b

0.02

Medium alone

133

109

82

Vehicle

121

97

80

Coculture

125

110

88b

0.2

Medium alone

121

76

63

Vehicle

115

69

60

Coculture

120

82

68b

1

Medium alone

122

0

0c

Vehicle

119

0

0c

 

Coculture

128

55

43bc

  1. *Mouse embryos were grown on three different kinds of culture system. In Medium alone M199 (Gibco) media were used. In Vehicle, vehicle solutions (ethanol/methanol) were used. In co-culture, embryos were grown on human endometrial epithelial cells (hEECs).
  2. aThe percentages of blastocyst at 72hour of culture.
  3. bValues in coculture were significantly higher than those in medium alone, respectively: P < 0.05.
  4. cValues were significantly lower than those of each control(0 μg/ml), respectively: P < 0.05.