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Figure 2 | Journal of Occupational Medicine and Toxicology

Figure 2

From: Silencing of hepcidin enforces the apoptosis in iron-induced human cardiomyocytes

Figure 2

Comet assay: effect of silencing hepcidin on ferrous iron-induced cardiomyocyte apoptosis. siRNA2 was employed to knock-down hepcidin levels and siRNA1 was injected as a negative control. Cultured human cardiomyocytes, pretreated with ferrous iron (500 microg/mL, 18 h), were transfected with each siRNAs (20 μM, 48 h). DNA damage was then determined by the comet assay. Ferrous iron-free treated cells were used as the control.

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