Figure 3

Effects of ferrous iron on GATA-4 m-RNA and protein levels in cardiomyocytes. GATA-4 mRNA expression was decreased in a dose-dependent manner after incubation with ferrous iron (Figure 3a). Western blots of nuclear GATA-4 protein expression also showed a dose-dependent decrease in response to ferrous iron in cardiomyocytes (Figure 3b). Cultured human cardiomyocytes were treated with different concentrations of ferrous iron. Doxorubicin (DOXO, 0.5 μM) was used as a positive control. Data are normalized to β-actin, and are expressed as the mean ± SD of six independent experiments.