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Table 1 Effect of different doses of ferrous iron on apoptosis in cultured human cardiomyocytes

From: Silencing of hepcidin enforces the apoptosis in iron-induced human cardiomyocytes

Ferrous Iron (μg/ml)

% apoptosis (mean ± SD)

0

1.0 ± 2.2

10

1.1 ± 2.3

50

4.2 ± 2.1

100

7.6 ± 2.5

250

8.4 ± 2.3

500

11.2 ± 2.1

Positive control: DOXO (0.5 μM)

10.1 ± 2.2

  1. Positive control: medium + DOXO (0.5 μM): 10.1 ± 2.2%.
  2. Cultured human cardiomyocytes were incubated with medium alone or in combination with ferrous iron at the indicated concentrations for 18 h to reach the maximal effect of ferrous iron. Medium plus DOXO (0.5 μM) was used as a positive control. Following the maximal effect of ferrous iron, cells were stained with annexin V-FITC and apoptosis analysis was determined by FACS. Each value represents the mean ± SD from six independent experiments.
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Journal of Occupational Medicine and Toxicology

ISSN: 1745-6673