Fig. 3
CCL9 is increased in response to HDE and CO2. Expression of CCL9 chemokine was examined in BAL (a) and serum (b) by ELISA. Only the lung showed significant increases in CCL9 in response to HDE. Increase of CO2 (5000 and 7500 ppm) induced what appeared to be a concentration-dependent increase in CCL9 production vs. ambient air (air) in the lung that was absent in serum as well. Expression in lung was confirmed by mRNA expression (c) which showed a similar pattern of CCL9 induction to barn dust and CO2. Bar graphs represent standard deviation with error bars shown (N = 6-8 mice/group). Statistical significance denoted by asterisks (*p < 0.05, **p < 0.001, ***p < 0.0001)