Fig. 2

Three dimensional detection and quantitative image analysis of viability staining of long-term cultured human PCLS. Human lung slices were stained with Calcein AM (yellow) and Ethidium homodimer 1 (EthD-1; red). Three stacks per slice of 30 μm thickness were taken randomly at days 1, 7 and 15. One representative images for each time point is shown. The stacks were analysed using IMARIS 5.5.3. software and were quantitatively evaluated as the number of red nuclei of dead cells (spots) per 10⁶ μm³ volume of yellow vital tissue Triton X-100 served as a dead cell control. Data are presented as Box and Whiskers, **p < 0.01 compared to day 1, Mann-Whitney test (n = 6_{(day 1, 7, Triton)}, n = 4_(day15))