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Table 5 Reduction in FemB copies from viable S. aureus still present on outer shell fabrics after washing with sanitizer

From: Survival of Staphylococcus aureus on the outer shell of fire fighter turnout gear after sanitation in a commercial washer/extractor

Duration of vortexing in water or sanitizer

Swatch type

Exp. no.

Inoculum (femB copies/ swatch)

femB copies from viable cells remaining on water washed swatch (femB /aSD)

femB copies from viable cells remaining on sanitizer washed swatch (femB /aSD)

Percent reduction

10 Seconds (Carrier Count Control)

Gemini™

1

2.2 × 106

1.0 × 105 /1.5 × 105

3.1 × 104 /8.0 × 104

69

Gemini™

2

2.0 × 106

3.6 × 104 /1.5 × 104

5.7 × 103 /4.7 × 103

84

Gemini™

3

2.0 × 107

5.6 × 103 /3.5 × 103

2.0 × 101 /4.4 × 101

99.6

Gemini™

4

6.0 × 106

5.4 × 104 /4.6 × 104

1.2 × 103 / 8.9 × 102

98

Advance™

1

1.0 × 107

4.8 × 104 /5.0 × 104

4.5 × 101 /2.2 × 101

100

Advance™

2

1.0 × 107

8.2 × 104 /4.7 × 104

3.7 × 103 /5.2 × 103

95

Pioneer™

1

2.2 × 105

2.8 × 104 /2.3 × 104

2.9 × 101 /4.7 × 101

99.9

Cotton

1

1.1 × 105

2.3 × 104 /2.3 × 104

1.6 × 102 /3.0 × 102

99.3

10 Minutes (Carrier Count Control)

Gemini™

1

1.1 × 107

6.7 × 104 /5.0 × 104

8.6 × 103 /9.2 × 103

87

Gemini™

2

2.3 × 108

7.2 × 105 /4.6 × 105

1.7 × 105 /2.7 × 105

77

Gemini™

3

4.0 × 107

8.1 × 104 /2.0 × 104

1.6 × 104 /1.8 × 104

80

Gemini™

4

1.5 × 108

9.7 × 104 /2.0 × 104

2.5 × 104 /3.8 × 104

74

Advance™

1

1.2 × 107

6.4 × 104 /2.5 × 105

4.7 /9.7

100

Advance™

2

5.5 × 106

7.6 × 104 /4.3 × 105

9.3 × 102 /1.2 × 104

99

Pioneer™

1

1.1 × 107

2.5 × 105 /1.1 × 105

1.0 × 103 /3.5 × 102

99.6

Cotton

1

9.1 × 106

2.1 × 106 /4.0 × 105

1.9 × 102 /1.7 × 102

100

Washer/extractor

Swatch type

Exp. no.

Inoculum (femB copies/Swatch)

femB copies from viable cells remaining on unwashed swatch (femB /aSD

femB copies from viable cells remaining on fabric sanitizer washed swatch (femB /aSD)

Percent reduction

Test 1

Gemini™

1

9.6 × 107

1.6 × 106 /1.7 × 106

6.8 × 103 /2.0 × 104

99.6

Test 2

Pioneer™

2

8.7 × 107

4.7 × 105 /2.1 × 106

3.1 × 102 / 2.0 × 104

99.9

  1. Carrier count swatches were vortex-washed for 10 s or 10 min, and then incubated in DEB for 5 h to elute remaining cells
  2. All carrier count experiments are average of 3 replicate swatches per experiment
  3. Washer/extractor test of S. aureus inoculated Gemini swatches are average of 4 swatches pinned to two turnout gear jackets
  4. Washer/extractor test of S. aureus inoculated Pioneer swatches are average of 3 swatches pinned to two turnout gear jackets
  5. aSD standard deviation