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Table 5 Reduction in FemB copies from viable S. aureus still present on outer shell fabrics after washing with sanitizer

From: Survival of Staphylococcus aureus on the outer shell of fire fighter turnout gear after sanitation in a commercial washer/extractor

Duration of vortexing in water or sanitizer Swatch type Exp. no. Inoculum (femB copies/ swatch) femB copies from viable cells remaining on water washed swatch (femB /aSD) femB copies from viable cells remaining on sanitizer washed swatch (femB /aSD) Percent reduction
10 Seconds (Carrier Count Control) Gemini™ 1 2.2 × 106 1.0 × 105 /1.5 × 105 3.1 × 104 /8.0 × 104 69
Gemini™ 2 2.0 × 106 3.6 × 104 /1.5 × 104 5.7 × 103 /4.7 × 103 84
Gemini™ 3 2.0 × 107 5.6 × 103 /3.5 × 103 2.0 × 101 /4.4 × 101 99.6
Gemini™ 4 6.0 × 106 5.4 × 104 /4.6 × 104 1.2 × 103 / 8.9 × 102 98
Advance™ 1 1.0 × 107 4.8 × 104 /5.0 × 104 4.5 × 101 /2.2 × 101 100
Advance™ 2 1.0 × 107 8.2 × 104 /4.7 × 104 3.7 × 103 /5.2 × 103 95
Pioneer™ 1 2.2 × 105 2.8 × 104 /2.3 × 104 2.9 × 101 /4.7 × 101 99.9
Cotton 1 1.1 × 105 2.3 × 104 /2.3 × 104 1.6 × 102 /3.0 × 102 99.3
10 Minutes (Carrier Count Control) Gemini™ 1 1.1 × 107 6.7 × 104 /5.0 × 104 8.6 × 103 /9.2 × 103 87
Gemini™ 2 2.3 × 108 7.2 × 105 /4.6 × 105 1.7 × 105 /2.7 × 105 77
Gemini™ 3 4.0 × 107 8.1 × 104 /2.0 × 104 1.6 × 104 /1.8 × 104 80
Gemini™ 4 1.5 × 108 9.7 × 104 /2.0 × 104 2.5 × 104 /3.8 × 104 74
Advance™ 1 1.2 × 107 6.4 × 104 /2.5 × 105 4.7 /9.7 100
Advance™ 2 5.5 × 106 7.6 × 104 /4.3 × 105 9.3 × 102 /1.2 × 104 99
Pioneer™ 1 1.1 × 107 2.5 × 105 /1.1 × 105 1.0 × 103 /3.5 × 102 99.6
Cotton 1 9.1 × 106 2.1 × 106 /4.0 × 105 1.9 × 102 /1.7 × 102 100
Washer/extractor Swatch type Exp. no. Inoculum (femB copies/Swatch) femB copies from viable cells remaining on unwashed swatch (femB /aSD femB copies from viable cells remaining on fabric sanitizer washed swatch (femB /aSD) Percent reduction
Test 1 Gemini™ 1 9.6 × 107 1.6 × 106 /1.7 × 106 6.8 × 103 /2.0 × 104 99.6
Test 2 Pioneer™ 2 8.7 × 107 4.7 × 105 /2.1 × 106 3.1 × 102 / 2.0 × 104 99.9
  1. Carrier count swatches were vortex-washed for 10 s or 10 min, and then incubated in DEB for 5 h to elute remaining cells
  2. All carrier count experiments are average of 3 replicate swatches per experiment
  3. Washer/extractor test of S. aureus inoculated Gemini swatches are average of 4 swatches pinned to two turnout gear jackets
  4. Washer/extractor test of S. aureus inoculated Pioneer swatches are average of 3 swatches pinned to two turnout gear jackets
  5. aSD standard deviation