Fig. 4

ECIS real-time monitoring of NuLi-1 cells in the presence of bacterial culture supernatants. Collected supernatants of the bacteria E.faecalis (a), A.radioresistens (b), S.pneumoniae (c), A.viridans (d) and A.hydrophila (e) were applied at four different concentrations (percentage of the culture supernatant in 100 μl sample) to NuLi-1 cells and their effects were measured over 48 h. Averages of normalized impedance differences between supernatant exposed data and control data are shown from 3 measured biological replicates. Cells exposed to bacterial growth medium THY were used as controls. Impedance was normalized to values at the time of supernatant addition