In vitro generation of reactive oxygen and nitrogen species by neutrophils in contact with PM
. A. In vitro production of reactive oxygen and nitrogen species by neutrophils from healthy volunteers (NHV) compared with neutrophils from asthmatic patients (NAP), measured by luminol-enhanced chemiluminescence and expressed as the area under the curve (AUC). The graph represents the mean of AUC for each group. B. Each line represents the chemiluminescence response of each subject that participated in the study, before and after treatment with PM2.5. The pattern shows a general increase in this response in the NAP group.