Fig. 7

Bio-plex ELISA of mouse bronchoalveolar lavage. BAL was collected by washing lungs 3 times with cold HEPES buffer from exposed and unexposed WT and TLR9^−/− mice (n = 6). Samples were centrifuged briefly to remove cells and fluid decanted. ELISA was done with antibodies to IL-1β, IL-6, IL-10, IL-12, IFNγ, and TNFα. TNFα (a) was significantly reduced after 5 days exposure in TLR9^−/− mice compared to WT (* = p ≤ 0.06). IFNγ (b) was significantly reduced after 5 and 20 days exposure in TLR9^−/− mice compared to WT (** = p ≤ 0.05)