Fig. 1

Metabolic activity of long-term cultured human PCLS. Metabolic activity in human PCLS was determined by the WST-1 assay. The negative control Triton X-100, a commonly used detergent, decreased metabolic activity in human PCLS to an OD corresponding to background (~0.04). Data are presented as Box and Whiskers, Mann-Whitney test (WST-1: n = 8_{(day 1, 8, 15, Triton)}, n = 5_{(day 5)}, n = 6_{(day 12, 14)}). The dotted line marks the mean measured OD on day 1. PCLS = precision cut lung slices