Fig. 4

TLRs involved in histone H4 mediated expression of inflammatory cytokines. The blocking antibody (10 mg/L) against TLR1, TLR2, TLR4, or TLR6 was concurrently administered when pulmonary vascular endothelial cells were challenged by histone H4 (10 mg/L) for 12 h. The mRNA of TNFA and IL1B was measured by real-time qPCR (a, b), and protein was measured by western blot (c, d). N = 3 independent replicates for all groups. *p < 0.05, **p < 0.01 compared with the control group; ^#p < 0.05, ^##p < 0.01 compared with the histone H4 group