Lung inflammation following a single exposure to swine barn air
© Gamage et al; licensee BioMed Central Ltd. 2007
Received: 29 July 2007
Accepted: 18 December 2007
Published: 18 December 2007
Exposure to swine barn air is an occupational hazard. Barn workers following an eight-hour work shift develop many signs of lung dysfunction including lung inflammation. However, the in situ cellular and molecular mechanisms responsible for lung dysfunction induced following exposure to the barn air remain largely unknown. Specifically, the recruitment and role of pulmonary intravascular monocytes/macrophages (PIMMs), which increase host susceptibility for acute lung inflammation, remain unknown in barn air induced lung inflammation. We hypothesized that barn exposure induces recruitment of PIMMs and increases susceptibility for acute lung inflammation with a secondary challenge.
Sprague-Dawley rats were exposed either to the barn or ambient air for eight hours and were euthanized at various time intervals to collect blood, broncho-alveolar lavage fluid (BALF) and lung tissue. Subsequently, following an eight hour barn or ambient air exposure, rats were challenged either with Escherichia coli (E. coli) lipopolysaccharide (LPS) or saline and euthanized 6 hours post-LPS or saline treatment. We used ANOVA (P < 0.05 means significant) to compare group differences.
An eight-hour exposure to barn air induced acute lung inflammation with recruitment of granulocytes and PIMMs. Granulocyte and PIMM numbers peaked at one and 48 hour post-exposure, respectively.
Secondary challenge with E. coli LPS at 48 hour following barn exposure resulted in intense lung inflammation, greater numbers of granulocytes, increased number of cells positive for TNF-α and decreased amounts of TGF-β2 in lung tissues. We also localized TNF-α, IL-1β and TGF-β2 in PIMMs.
A single exposure to barn air induces lung inflammation with recruitment of PIMMs and granulocytes. Recruited PIMMs may be linked to more robust lung inflammation in barn-exposed rats exposed to LPS. These data may have implications of workers exposed to the barn air who may encounter secondary microbial challenge.
Swine production is a major agricultural business in North America [1–3]. These days thousands of pigs are raised in large confinement buildings compared to small-scale family-managed operations in the past. Modern pig production operations increasingly employ full-time workers who might spend up to 8 hours/day inside the barn compared to 1–2 hours of work on the small farm in the past [4–6]. Although the environment inside these confinement buildings appears to be clean, it contains high levels of endotoxins, dust, bacterial DNA and gases such as ammonia and hydrogen sulfide [7–9].
There are evidences for reduced forced expiratory volume in one second (FEV1), wheeze and increased airway hyperresponsiveness (AHR) following a single exposure of naïve workers/volunteers to the barn air [10–12]. Sputum and bronchoalveolar lavage (BAL) obtained after a three hour exposure to the barn air showed increased levels of interleukin (IL)-8, IL-6, tumor necrosis factor-α (TNF-α), fibronectin and albumin [13–15]. There was also a 75-fold increase in the neutrophils and 2–3 fold increase in mononuclear cells in BAL from human naive volunteers exposed to the barn air for 3–5 hours while leukocyte counts increased in peripheral blood within 6 hours of an exposure . Recent in vitro data show that swine barn dust stimulates secretion of IL-1β and TNF-α from alveolar macrophages and epithelial cells and expression of adhesion molecules on epithelial cells . Recently, we have used a rat model to report that a single exposure to the barn air induces lung inflammation and AHR . However, it remains unknown if an exposure to the barn air alters lung susceptibility to secondary challenges with microbes or their components such as lipopolysaccharide (LPS).
Lung inflammation is characterized by recruitment of neutrophils and monocytes into the alveolar septa and air spaces . Monocyte recruitment into alveolar spaces in inflamed lungs is important for the removal of inflammatory debris and repair of tissue damage. However, prior to the entry of monocytes into alveolar spaces, they undergo physical interaction with the endothelium of lung capillaries and consequently may be retained in the capillaries. This is supported by our previous observations on the transient recruitment of pulmonary intravascular monocytes/macrophages (PIMMs) in lung inflammation in a rat model of sepsis [19, 20]. We have also shown that PIMMs, similar to resident pulmonary intravascular macrophages in some domestic animal species, may alter the lung responses to a subsequent challenge [21–23, 20]. Currently, there are no data on the recruitment and biology of PIMMs in animals exposed to the barn air.
Considering the putative critical roles of PIMMs, we undertook a series of in vivo studies to characterize PIMM recruitment and their functions in lung inflammation induced following exposure to the barn air. The data from these experiments demonstrate recruitment of neutrophils and PIMMs following a single exposure to the barn air and show a linkage between recruited PIMMs and increased lung inflammation following a secondary challenge with E. coli LPS.
The University of Saskatchewan Committee on Animal Care Assurance approved protocols for the use of experimental animals in this study. Sprague-Dawley rats were kept in a swine barn for 8 hours. The cages were hung from the barn ceiling at a height of 5 feet from the floor. The rats were taken out of the barn at the end of the exposure and maintained in ambient air until euthanasia.
Experiment 1: Effect of exposure to the swine barn air
Rats (N = 25) exposed to the barn air were euthanized 1 hour, 24, 48, 72 and 120 hours post-exposure (n = 5 for each time point). Control rats (N = 5) were kept in clean air prior to euthanasia.
Experiment 2: Effect of secondary challenge with E. coli LPS on barn air exposed rats
Based on the data from Experiment 1, rats were administered either E. coli LPS (Sigma Chemical Co. St. Louis, MO, 1.5 μg/g body weight intravenous; n = 5) or saline (0.5 mL intravenous; n = 5) at 48 hours after an 8 hour exposure to the barn air. Unexposed rats were treated with E. coli LPS (n = 5) or saline (n = 5).
Blood and broncho-alveolar lavage fluid (BALF) collection and analyses
At the end of the designated time points in Experiment 1 and 2, rats were euthanized (1 mg xylazine and 10 mg ketamine/100 g) for collection of blood, BALF and lung samples. Blood was collected by cardiac puncture for differential and total leukocyte counts. BALF was collected by lavaging the whole lung with three mL of ice cold Hanks Balanced Salt Solution (Sigma Chemicals Co., St. Louis, MO). The BALF was stored on ice until processing for total and differential leukocyte counts.
Lung tissue processing
Left lung was snap frozen in liquid nitrogen, stored at -80°C and was later used in ELISA.
Right lobes of the lung were fixed in situ by instilling 4% paraformaldehyde in phosphate buffered saline (0.0016 M NaH2PO4, 0.008 M Na2HPO4 and 0.15 M NaCl), pH 7.2 for 30 minutes followed by immersion in the same fixative for 16 hours at 4°C. Three pieces collected from right lung were dehydrated, and embedded in paraffin. Five to seven μm thick sections were prepared and placed on glass slides coated with Vectabond (Vector Labs) and incubated at 55°C for 30 minutes to increase adherence of sections. Lung sections were stained with hematoxylin and eosin for histopathological assessment.
Immuno-histochemistry and cell counts
Lung sections were processed for immunohistochemistry as described previously . Briefly, sections were deparaffinized, dehydrated, treated with hydrogen peroxide (5% in methanol) to neutralize endogenous tissue peroxidase and exposed to pepsin (2 mg/ml 0.01 N HCl) to unmask the antigens. The sections were incubated with primary antibodies against monocytes/macrophages (ED-1, 1:75; Serotec, USA), granulocytes (HIS-48 1:50; BD Bioscience Canada), IL-1β (1:100; Santa Cruz Biotechnology, Inc., USA), TNF-α and TGF-β2 (1:75; R&D Systems, Inc., USA) for 60 minutes followed by incubation with appropriate secondary antibodies conjugated with horseradish peroxidase (1:100–1:250) for 30 minutes. Controls included staining without primary antibody or anti-von Willebrand Factor (vWF) antibody, which recognizes vascular endothelium, or with isotype-matched immunoglobulins. These sections were counter-stained with methyl green and immunohisotchemically positive cells in the lung septum were counted in 10 high power fields (400×; 0.096 mm2 per field) under oil-immersion objective by a person blinded to the design of the experiment.
Lungs samples were prepared for immuno-electron microscopy as described previously . Briefly, tissues fixed in 0.1% glutaraldehyde and 2.0% paraformaldehyde in 0.1 M sodium cacodylate buffer for 3 hours at 4°C, dehydrated and infiltrated with LR White resins. The tissues were polymerized under ultraviolet light at -8°C for 3 days. Semi-thin (1 mm) sections were prepared to select areas for ultrathin (100 nm) sections. Sections were stained with ED-1 (1:100), IL-1β (1:25), TNF-α (1: 25) and TGF-β2 (1:25) antibodies followed by appropriate gold-conjugated secondary antibodies (respective, 1:100 diluted) and examined in an electron microscope at 60 kV. Immuno-electron microscopy controls included omission of primary antibody or staining of lung sections with anti-von Willebrand Factor antibody.
Enzyme-linked immunosorbent linked assay
Lung samples were homogenized in Hank's balanced salt solution (HBSS) containing protease inhibitor cocktail (100 μl/10 ml; Sigma-Aldrich Co, MO, USA) in a ratio of 0.1 g of tissue in 1 ml of the solution, and centrifuged at 25,000 rpm at 4°C to collect the supernatant which was stored at -70°C in 100 μl aliquots. The ELISA kits for rat IL-1β, TNF-α and TGF-β2 were purchased from R & D systems, Inc., USA. Microtiter plates (Immulon 4 HBX, VWR CAN LAB, Canada) were coated with 100 μL of capture antibody and incubated overnight at room temperature. Non-specific bindings were blocked with 200 μL of 1% BSA. Then, standards and the samples in 100 μL quantities were incubated for 2 hours at room temperature. This was followed by incubation with 100 μL of biotinylated detecting antibodies for 2 hours at room temperature. Subsequently, plates were incubated with 100 μL of avidin-HRP (Vector laboratories, Inc., USA) for 40 minutes at room temperature. Finally, 100 μL of 3,3', 5,5'-tetramethyl-benzidine dihydrochloride (TMB) substrate (Mandel Scientific, ON, Canada) was added and incubated for 10–15 minutes at room temperature. After adequate color development, the reaction was stopped with 50 μl of 1 M sulfuric acid. In between each step until adding the substrate, plates were washed with PBS containing 0.05%-Tween20 (PBST). The optical densities were measured at 450 nm. Cytokine concentrations of test samples were determined using linear regression of standard curve and expressed as picograms per milliliter. The optimal concentrations for capture (TNF-α: 1 μg/ml, IL-1β:1 μg/ml and TGF-β2: 2 μg/ml) and detecting antibody (TNF-α: 1 μg/ml, IL-1β: 300 ng/ml and TGF-β2: 50 ng/ml) pair for each cytokine were titrated prior to running ELISA with test samples.
All values were presented as mean ± standard error (SE). We performed one-way ANOVA to compare granulocyte and ED-1 positive macrophage numbers at various time points following exposure to barn air or ambient air (control). Using two-way ANOVA, we examined the effect of exposure (barn or ambient air), effect of secondary challenge (saline or LPS) and the interaction effect between exposure and secondary challenge. ANOVA was followed by Tukey's post-hoc test (SigmaStat®, version 2.0 for Windows® 95, NT and 3.1, 1997; Chicago, IL, USA). Statistical significance was accepted at P < 0.05.
There were no differences in total and differential leukocyte counts among various groups in this study (P > 0.05; data not shown).
Recruitment of granulocytes and PIMMs
Response to secondary challenge
Increase in granulocytes following secondary challenge
Expression and quantification of IL-1β
Expression and quantification of TNF-α
Expression and quantification of TGF-β2
The data reported in this paper show that a single exposure to the barn air induces acute lung inflammation including recruitment of granulocytes and PIMMs. The data further show that exposure to the barn air increases susceptibility for increased lung inflammation following a secondary challenge, which may be partially due to recruited PIMMs.
Although it has been known for some time that swine barn workers experience acute lung dysfunction including reduction in FEV1 and inflammation across a single shift in the barn [26–28], there has been a lack of reliable animal models to study cellular and molecular changes upon exposure to the barn air. Recently, we reported characterization of a rat model to investigate the pulmonary impact of exposure to the barn air . Now, we have used this model to examine lung inflammation at various times points following a single 8 hour exposure to the barn air. It is well established that pig barn air contains significant amounts of endotoxin, dust and gases such as ammonia [29, 30]. The data showed significant increases in granulocyte numbers in the lung septum and the bronchiolar walls (Data not shown) at one hour following the 8 hour exposure to barn air. Recruitment of granulocytes in lungs of barn exposed rats is consistent with the recently reported production of IL-8, a potent chemoattractant for granulocytes, by airway epithelial cells exposed to the barn dust in vitro . Furthermore, BAL fluid collected from human workers following a single 8 hour shift contains higher levels of IL-8 . Therefore, expression of chemoattractants such as IL-8 following exposure to the barn air may be instrumental in provoking recruitment of granulocytes and induction of acute lung inflammation following a single exposure to pig barn air.
Neutrophil migration is typically followed by recruitment of monocytes/macrophages. We observed a novel increase in ED-1 positive monocytes/macrophages cells in the alveolar septa at 48 hours after barn exposure compared to other time points. ED-1 antibody recognizes a lysosomal protein and has previously been used to recognize rat monocytes/macrophages [32–34]. Because of resolution limits of light microscopy, we used immuno-electron microscopy to confirm the intravascular location of ED-1 positive monocytes/macrophages. The recruitment pattern of PIMMs in barn-exposed rats determined through ED-1 staining is similar to that observed following a single bacterial challenge [19, 20]. However, transient PIMM recruitment induced by barn air is different from more permanent PIMM accumulation observed following bile duct ligation . Because we did not observe any changes in BAL neutrophil and monocyte/macrophage cell counts, it appears that exposure to barn air predominantly induced vascular accumulation of neutrophils and PIMMs. It is also possible that a strong enough chemotactic gradient was not induced following a single exposure to barn air. Nevertheless, the data show a single cycle of acute lung inflammation is induced following an 8 hour exposure pig barn air.
We also examined the response of barn exposed rats specifically in the context of recruited PIMMs to a secondary challenge. This was necessitated because resident pulmonary intravascular macrophages in cattle, sheep and horses are credited with induction of robust lung inflammation [36, 22, 37]. Furthermore, we have recently reported that PIMMs recruited following an intraperitoneal injection of E. coli bacteria alter lung susceptibility to a secondary challenge with E. coli LPS . Our data show that LPS treatment of barn-exposed rats compared to normal rats resulted in a higher accumulation of granulocytes and increased number of cells positive for TNF-α but not IL-1β in the lungs. Interestingly, ELISA did not reveal differences among any of the groups for concentration of IL-1β and TNF-α in lung tissues. We do not know the reasons for the discrepancy between histologic and ELISA results for TNF-α. Differences in sensitivities of the two methods could be a contributing factor. Immuno-histochemistry may have detected the residual intracellular cytokines while most of the cytokines were secreted into the circulation thus resulting in lack of differences between groups with ELISA. We still believe that ELISA is a more powerful and sensitive method for molecular quantification. It is possible that we may have missed the window of increased concentrations of the assayed cytokines in rat lungs. Nevertheless, it is important to note that granulocyte numbers were higher in LPS-treated rats that contained PIMMs. Because granulocyte migration requires vascular expression of cytokines and adhesion molecules and is based on the localization of IL-1β and TNF-α in PIMMs, we believe that recruited PIMMs may have played a major role in provoking increased migration of granulocytes into inflamed lungs.
Inflammation is manifested through a complex interplay of inflammatory and pro-inflammatory cytokines. Therefore, we also examined the expression of TGF-β2, which is classified as an anti-inflammatory cytokine involved in tissue repair and remodeling [38–42]. We noticed highest lung expression of TGF-β2 in conjunction with peak recruitment of PIMMs at 48 hours after exposure to the barn. Interestingly, rats treated with LPS at 48 hours post-exposure or without barn exposure showed reduced expression of TGF-β2. These data suggest that TGF-β2 may play anti-inflammatory roles in lung inflammation induced following exposure to the barn air, and that its expression may be suppressed to manifest acute inflammation engendered through LPS treatment of the exposed rats. PIMMs showed TGF-β2 in addition to IL-1β and TNF-α to underscore the complex and multifaceted roles of monocytes/macrophages in lung inflammation. It appears that the relative balance of cytokines produced by monocytes/macrophages results in fine and tight regulation of inflammatory processes.
We report novel recruitment of PIMMs in barn-exposed rats and increased lung inflammation in exposed rats subjected to a secondary challenge with LPS. It appears that recruited PIMMs may be involved in increased inflammation through their contribution of multiple cytokines.
The work was supported through a grant from Lung Association of Saskatchewan and Natural Sciences and Engineering Research Council of Canada to Dr. B. Singh. Dr. Charavaryamath is supported through Graduate Merit Scholarship from University of Saskatchewan (2002–2004), Founding chairs graduate scholarship from Institute of Agricultural, Rural and Environmental Health (I.ARE.H) and a scholarship from the CIHR Strategic Training Program in Public Health and the Agricultural Rural Ecosystem (PHARE) and Partner Institutes including the Institute of Cancer Research, Institute of Circulatory and Respiratory Health, Institute of Infection and Immunity, Institute of Population and Public Health and the University of Saskatchewan.
- Hog Statistics (http://www.statcan.ca/english/freepub/23–010-XIE/23–010-XIE2005004.pdf) Statistics Canada, Agriculture Divison; 2006.Google Scholar
- Farm operators by farm type, by province ( 2001 Census of Agriculture) ( Canada)2001. [http://www40.statcan.ca/l01/cst01/agrc22a.htm]
- World Pork Meat and Swine Trade Overview2006. [http://www.fas.usda.gov/dlp/circular/2005/05–04LP/porkoverview.pdf]
- Wenger I: Air Quality and Health of Career Pig Barn Workers. Advances in Pork Production 1999, 10: 93–101.Google Scholar
- Wenger II, Ouellette CA, Feddes JJ, Hrudey SE: The design and use of the Personal Environmental Sampling Backpack (PESB II) for activity-specific exposure monitoring of career pig barn workers. J Agric Saf Health 2005, 11: 315–324.View ArticlePubMedGoogle Scholar
- Cole D, Todd L, Wing S: Concentrated swine feeding operations and public health: a review of occupational and community health effects. Environ Health Perspect 2000, 108: 685–699. 10.2307/3434721PubMed CentralView ArticlePubMedGoogle Scholar
- Asmar S, Pickrell JA, Oehme FW: Pulmonary diseases caused by airborne contaminants in swine confinement buildings. Vet Hum Toxicol 2001, 43: 48–53.PubMedGoogle Scholar
- Donham KJ, Popendorf W, Palmgren U, Larsson L: Characterization of dusts collected from swine confinement buildings. Am J Ind Med 1986, 10: 294–297. 10.1002/ajim.4700100318View ArticlePubMedGoogle Scholar
- Cormier Y, Israel-Assayag E, Racine G, Duchaine C: Farming practices and the respiratory health risks of swine confinement buildings. Eur Respir J 2000, 15: 560–565. 10.1034/j.1399-3003.2000.15.22.xView ArticlePubMedGoogle Scholar
- Cormier Y, Duchaine C, Israel-Assayag E, Bedard G, Laviolette M, Dosman J: Effects of repeated swine building exposures on normal naive subjects. Eur Respir J 1997, 10: 1516–1522. 10.1183/09031936.97.10071516View ArticlePubMedGoogle Scholar
- Senthilselvan A, Zhang Y, Dosman JA, Barber EM, Holfeld LE, Kirychuk SP, Cormier Y, Hurst TS, Rhodes CS: Positive human health effects of dust suppression with canola oil in swine barns. Am J Respir Crit Care Med 1997, 156: 410–417.View ArticlePubMedGoogle Scholar
- Palmberg L, Larssson BM, Malmberg P, Larsson K: Airway responses of healthy farmers and nonfarmers to exposure in a swine confinement building. Scand J Work Environ Health 2002, 28: 256–263.View ArticlePubMedGoogle Scholar
- Larsson BM, Palmberg L, Malmberg PO, Larsson K: Effects of exposure to swine dust on levels of IL-8 in airway lavage fluid. Thorax 1997, 52: 638–642.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang Z, Malmberg PO, Larsson P, Larsson BM, Larsson K: Time course of interleukin-6 and tumor necrosis factor-a increase in serum following inhalation of swine dust. Am J Respir Crit Care Med 1996, 153: 147–152.View ArticlePubMedGoogle Scholar
- Larsson K, Eklund AG, Hansson LO, Isaksson BM, Malmberg PO: Swine dust causes intense airways inflammation in healthy subjects. Am J Respir Crit Care Med 1994, 150: 973–977.View ArticlePubMedGoogle Scholar
- Wang Z, Malmberg PO, Ek A, Larsson K, Palmberg L: Swine dust induces cytokine secretion from human epithelial cells and alveolar macrophages. Clin Exp Immunol 1999, 115: 6–12. 10.1046/j.1365-2249.1999.00776.xPubMed CentralView ArticlePubMedGoogle Scholar
- Charavaryamath C, Janardhan KS, Townsend HG, Willson P, Singh B: Multiple exposures to swine barn air induce lung inflammation and airway hyper-responsiveness. Respir Res 2005, 6: 50. 10.1186/1465-9921-6-50PubMed CentralView ArticlePubMedGoogle Scholar
- Chignard M, Balloy V: Neutrophil recruitment and increased permeability during acute lung injury induced by lipopolysaccharide. Am J Physiol Lung Cell Mol Physiol 2000, 279: L1083-L1090.PubMedGoogle Scholar
- Singh B, Doane KJ, Niehaus GD: Ultrastructural and cytochemical evaluation of sepsis-induced changes in the rat pulmonary intravascular mononuclear phagocytes. J Anat 1998, 192: 13–23. 10.1046/j.1469-7580.1998.19210013.xPubMed CentralView ArticlePubMedGoogle Scholar
- Charavaryamath C, Janardhan KS, Caldwell S, Singh B: Pulmonary intravascular monocytes/macrophages in a rat model of sepsis. Anat Rec A Discov Mol Cell Evol Biol 2006, 288A: 1259–1271. 10.1002/ar.a.20401View ArticleGoogle Scholar
- Staub NC: Pulmonary intravascular macrophages. Ann Rev Physiol 1994, 56: 47–67. 10.1146/annurev.ph.56.030194.000403View ArticleGoogle Scholar
- Singh B, Pearce JW, Gamage LN, Janardhan K, Caldwell S: Depletion of pulmonary intravascular macrophages inhibits acute lung inflammation. Am J Physiol Lung Cell Mol Physiol 2004, 286: L363-L372. 10.1152/ajplung.00003.2003View ArticlePubMedGoogle Scholar
- Parbhakar OP, Duke T, Townsend HG, Singh B: Depletion of pulmonary intravascular macrophages partially inhibits lipopolysaccharide-induced lung inflammation in horses. Vet Res 2005, 36: 557–569. 10.1051/vetres:2005016View ArticlePubMedGoogle Scholar
- Janardhan KS, Appleyard GD, Singh B: Expression of integrin subunits alphav and beta3 in acute lung inflammation. Histochem Cell Biol 2004, 121: 383–390. 10.1007/s00418-004-0649-1View ArticlePubMedGoogle Scholar
- Singh B, Rawlings N, Kaur A: Expression of integrin avb3 in pig, dog and cattle. Histol Histopath 2001, 16: 1037–1046.Google Scholar
- Senthilselvan A, Dosman JA, Kirychuk SP, Barber EM, Rhodes CS, Zhang Y, Hurst TS: Accelrated lung function decline in swine confinement workers. Chest 1997, 111: 1733–1741.View ArticlePubMedGoogle Scholar
- Kirychuk SP, Senthilselvan A, Dosman JA, Zhou C, Barber EM, Rhodes CS, Hurst TS: Predictors of longitudinal changes in pulmonary function among swine confinement workers. Can Respir J 1998, 5(6):472–478.PubMedGoogle Scholar
- Schenker MB, Christiani D, Cormier Y, Dimich-Ward H, Doekes G, Dosman JA: Respiratory health hazards in agriculture. Volume 158. Edited by: Schenker MB. American Thoracic Society; 1998:S1-S76.Google Scholar
- Cormier Y, Tremblay GM, Mariaux A, Brochu G, Lavoie JP: Airborne microbial contents in two types of swine confinement buildigns in Quebec. Am Ind Hyg Assoc J 1990, 51: 304–309.View ArticlePubMedGoogle Scholar
- Reynonlds SJ, Donham KJ, Whitten P, Merchant JA, Burmeister LF, Popendorf WJ: Longitudinal evaluation of dose-response relationships for environmental exposures and pulmonary function in swine production workers. Am J Ind Med 1996, 29: 33–40. Publisher Full Text 10.1002/(SICI)1097-0274(199601)29:1%3C33::AID-AJIM5%3E3.0.CO;2-#View ArticleGoogle Scholar
- Romberger DJ, Bodlak V, von Essen SG, Mathisen T, Wyatt TA: Hog barn dust extract stimulates IL-8 and IL-6 release in human bronchial epithelial cells via PKC activation. J Appl Physiol 2002, 93: 289–296.View ArticlePubMedGoogle Scholar
- Sminia T, Dijkstra CD: The origin of osteoclasts: an immunohistochemical study on macrophages and osteoclasts in embryonic rat bone. Calcif Tissue Int 1986, 39: 263–266. 10.1007/BF02555216View ArticlePubMedGoogle Scholar
- Dijkstra CD, Dopp EA, Joling P, Kraal G: The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in rat recognized by monoclonal antibodies ED1, ED2 and ED3. Adv Exp Med Biol 1985, 186: 409–419.PubMedGoogle Scholar
- Damoiseaux JG, Dopp EA, Calame W, Chao D, MacPherson GG, Dijkstra CD: Rat macrophage lysosomal membrane antigen recognized by monoclonal antibody ED1. Immunology 1994, 83: 140–147.PubMed CentralPubMedGoogle Scholar
- Chang SW, Ohara N: Chronic biliary obsrtuction induces pulmonary intravascular phagocytosis and endotoxin sensitivity in rats. J Clin Invest 1994, 94: 2009–2019.PubMed CentralView ArticlePubMedGoogle Scholar
- Singh B, de la Concha-Bermejillo A: Gadolinium chloride removes pulmonary intravascular macrophages and curtails the degree of ovine lentivirus-induced lymphoid interstitial pneumonia. Int J Exp Pathol 1998, 79(3):151–162.PubMedGoogle Scholar
- Sone Y, Nicolaysen A, Staub NC: Effect of particles on sheep lung hemodynamics parallels depletion and recovery of intravascular macrophages. J Appl Physiol 1997, 83: 1499–1507.PubMedGoogle Scholar
- Reidy MF, Wright JR: Surfactant protein A enhances apoptotic cell uptake and TGF-beta1 release by inflammatory alveolar macrophages. Am J Physiol Lung Cell Mol Physiol 2003, 285: L854-L861.View ArticlePubMedGoogle Scholar
- Ishii Y, Hashimoto K, Nomura A, Sakamoto T, Uchida Y, Ohtsuka M, Hasegawa S, Sagai M: Elimination of neutrophils by apoptosis during the resolution of acute pulmonary inflammation in rats. Lung 1998, 176: 89–98. 10.1007/PL00007597View ArticlePubMedGoogle Scholar
- Cox G: IL-10 enhances resolution of pulmonary inflammation in vivo by promoting apoptosis of neutrophils. Am J Physiol 1996, 271: L566-L571.PubMedGoogle Scholar
- Cox G, Crossley J, Xing Z: Macrophage engulfment of apoptotic neutrophils contributes to the resolution of acute pulmonary inflammation in vivo. Am J Respir Cell Mol Biol 1995, 12: 232–237.View ArticlePubMedGoogle Scholar
- Opal SM, DePalo VA: Anti-inflammatory cytokines. Chest 2000, 117: 1162–1172. 10.1378/chest.117.4.1162View ArticlePubMedGoogle Scholar
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